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. 2008 Aug 29;105(36):13604–13609. doi: 10.1073/pnas.0801854105

Fig. 3.

Fig. 3.

Effects of MTS reagents on WT and mutant GABAARs. (A) Structures and lengths of the MTS reagents that covalently modify an introduced cysteine and representative current traces from two different oocytes expressing αβK196Cγ receptors before and after modification by charged MTS reagents are shown. Modification of αβK196Cγ by a 2-min application of 2 mM MTSET+ (arrow) enhances EC30–60 GABA current amplitude whereas modification using a 2-min application of 2 mM MTSES− (arrow) decreases EC30–60 GABA current amplitude. (B) Bar graph summary of the percentage of change (mean ± SEM) in low (EC30–60) and max GABA current (IGABA) amplitude after modification of WT and mutant (βE153C and βK196C) receptors with MTSET or MTSES. The percentage of change in IGABA after MTS treatment is defined as: [((Iafter/Iinital) − 1) × 100]. Negative values represent a decrease in IGABA after MTS reaction, whereas positive values represent an increase in IGABA. *, values are significantly different from WT, P < 0.05 (one-way ANOVA).