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. 2008 Sep 2;105(36):13610–13613. doi: 10.1073/pnas.0802131105

Fig. 1.

Fig. 1.

Activity-induced prolonged epac1-camps response in synaptic boutons. (A) Pseudocolor YFP/CFP FRET ratio images of muscle 12 type III bouton expressing Epac1-cAMPs before (Con) and 5 s after 70-Hz stimulation for 5 s (Stim). (Size bar: 1 μm.) (B) Rapid decrease in YFP and increase in CFP fluorescence from a single type III bouton upon 5 s of activity (arrow). (C) Normalized YFP/CFP ratio time course shows a loss in FRET for the experiment in B. (Inset) No response is seen with direct excitation of YFP (n = 4). Error bars, which indicate SEM, are comparable in size to symbols. (D) Time course of epac1-camps response in muscle 6/7 type Ib glutamatergic boutons stimulated for 5 s at 70 Hz (n = 5).