Fig. 3.

A subset of insulin autoreactive CD8 T cells can be identified in some long-term diabetics (A) Insulin B10–18 tetramer positive CD8 frequency in a highly pure preparation of CD8 T cells from a long-term diabetic (age of onset 13 years, duration of diabetes 8 years) and a paired control. Both patient and control were HLA-A2.1+. Representative two-color dot plots are presented. (B) Insulin B10–18 tetramer positive CD8 frequency in long-term type 1 diabetics (n = 11) both positive and negative patients and controls. (C) Clinical characteristics of HLA-A2 positive type 1 diabetics with and without insulin B10–18 tetramer staining compared with matched controls.