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. 2008 Sep 3;105(36):13656–13661. doi: 10.1073/pnas.0803522105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 1. — BRET measurements of cAMP levels in living cells to monitor dopamine D2_L receptor activation. (A and B) Variations of cAMP levels in HEK293 cells stably expressing the EPAC biosensor and D2_LR. (A) Kinetics of cAMP variations after treatment of the cells at time 0 with forskolin (25 μM) in the absence (▵) or presence (■) of quinpirole 1 μM as indicated and the emission ratios (Rluc/YFP) were measured. Cells incubated in PBS (○) were used as a negative control. (B) Dose-response curve of quinpirole for inhibiting forskolin-stimulated cAMP accumulation in HEK cells. cAMP production was normalized to the percentage of forskolin-stimulated cAMP accumulation (set at 100%).