Fig. 6.

Comparison of H3K9 methylation and acetylation between jmj6-2 and WT. (A) Nuclei isolated from leaves of 10-day-old seedlings of WT (ZH11) and jmj6 mutants were immunostained with antibodies indicated on the left and revealed by Alexa Fluor 488. The same cells were stained by DAPI. (B) Relative intensity of the fluorescence was measured using the software Quantity-One 4.6 (BIO-RAD). Bars represent SD from measures of 18 nuclei for each antibody. Student's t tests reveal that the differences between ZH11 and jmj6–2 for H3K9me3, H3K9me2, and H3K9me1 were significant at 5%.