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. Author manuscript; available in PMC: 2009 Apr 14.
Published in final edited form as: Brain Res. 2008 Feb 19;1204:16–23. doi: 10.1016/j.brainres.2008.02.018

Figure 1. Ethanol augments apoptosis in fetal rhombencephalic neurons in vitro, and antioxidants prevent the pro-apoptotic effects of 75 mM ethanol.

Figure 1

Figure 1

Figure 1

Figures 1A and 1B show fetal rhombencephalic neurons stained with Hoechst 33342 from cultures that were maintained under control conditions (no ethanol) or that were treated for the last 24 hours (DIV5 to DIV6) with 75 mM ethanol (ethanol). Control and ethanol-treated neurons treated with EGCG (1C, 1D), curcumin (1E, 1F), resveratrol (1G, 1H), LA (1I, 1J), and melatonin (1K, 1L) are also depicted. Hoechst-stained living and apoptotic neurons were identified as described. A higher magnification (see inset) was used to identify neurons that exhibit the characteristics of apoptotic cells. Arrows point to one or a cluster of fragmented/apoptotic nuclei.