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. Author manuscript; available in PMC: 2008 Sep 10.
Published in final edited form as: Biochemistry. 2006 Dec 5;45(48):14325–14336. doi: 10.1021/bi0612059

Figure 4.

Figure 4

Acetyltransferase activity assay of At1g77540. At1g77540 displayed acetyltransferase activity on histones H3 and H4. Data were generated using a filter binding assay (29). 0.08 mg/mL enzyme was mixed with 1 mM DTT, 100 μM acetyl-CoA (4.95 μCi [3H]-acetyl-CoA), and 100 μM peptide substrate in 1× TBA buffer pH 7.0. Data were graphed as product formed vs. time, and a linear fit was applied to determine the rate. At1g77540 acetylates H3 (circles) at a rate of 0.04 nmol min−1 mg−1 of enzyme and H4 (triangles) at a rate of 0.015 nmol min−1 mg−1 of enzyme. The rate of non-enzymatic acetylation was determined from a matching reaction that contained enzyme buffer instead of enzyme. The background rate was determined and subtracted from the enzymatic rate. KaleidaGraph was used to analyze and plot the data.