Figure 5.

¹H-¹⁵N-HSQC spectrum of free and CoA-bound At1g77540. A, Spectrum of uniformly ¹⁵N-labeled At1g77540 recorded at 25°C in 10 mM KH₂PO₄, 50 mM KCl, 3 mM NaN3, and 90% H₂O/10% D₂O at pH 6.0. Assigned backbone amide resonance peaks are labeled with the single letter amino acid abbreviation and residue number corresponding to the primary sequence. Side chain indole resonances for Trp¹⁰ and Trp⁹⁰ are shown in the lower left hand corner of the spectrum. Horizontal lines in the upper right hand corner display positions of side-chain amide groups of residues (Asn¹¹, Asn³², and Asn³³). B, Overlay of ¹H-¹⁵N-HSQC spectra At1g77540 (red) and At1g77540–CoA complex (black). The most prominent chemical shift changes of residues affected by the addition of equimolar CoA to the protein solution are labeled as single letter abbreviation.