Figure 3.

Schematic representation of the construction of mutated and chimeric proteins. The full sequences of the cytosolic domains of both TGN38 and lgp120 are shown for the wild-type proteins as well as for the proteins with altered targeting signals. Tyrosine-containing steady-state localization signals are underlined, and the exchange of TGN38 and lgp120 targeting signals are depicted in large type. The nomenclature indicates either an L, for lgp120, or T, for TGN38, for the lumenal domains, TMDs, and cytoplasmic domains of each of the chimeric proteins.