Figure 6.

Addition of the full-length cytosolic domain of TGN38 to the lumenal domain and TMD of lgp120 is not sufficient to divert the chimeric protein to the TGN. HeLa cells stably transfected with the LLT construct were incubated in the presence of the mAb to lgp120 (A and C), and the intracellular localization was determined by indirect immunofluorescence. Colocalization with an endogenous lysosomal marker, cathepsin D (B) is shown, as well as the lack of overlap in distribution with endogenous TGN46 (D).