Table 1.
Oligonucleotides used in constructing lgp120 mutants and chimaeras
| Lumenal domain lgp120-5′: |
| GCAACCGCCGTCCTTCGGCCTCGGC |
| Lumenal domain lgp120-3′: |
| GGGGTCGACGCCGATGAGGTAGGCGATGAGGACGAT |
| Transmembrane domain lgp120-5′: |
| TTTGTCGACCTGATCCCCATTGCTGTGGGCGGG |
| Transmembrane domain lgp120-3′: |
| GGGGTCGACGCCGATGAGGTAGGCGATGAGGACGAT |
| Cytoplasmic domain lgp120-5′: |
| TTTGTCGACAGGAAGAGGAGTCACGCGGGCTATCAG |
| Cytoplasmic domain lgp120-3′: |
| GCCCTCGAGGCGCACCTGCCCACCAGGCTAGATGGT |
| lgp120-SDYQRL-3′: |
| CAGGCTAGAGGCGCTGATAGTCCGAGTGACTCCTC |
| Lumenal domain TGN38-5′: |
| AGGGACCAAGCTAGCATGCAGTTCCTGGTTCGGTTGCTC |
| Lumenal domain TGN38-3′: |
| GGGGTCGACGTAAGCAATATAGAGGACAGCAACAAG |
| Transmembrane domain TGN38-5′: |
| TTTGTCGACTTCTTTGCTTATCTGGTGACCGCT |
| Transmembrane domain TGN38-3′: |
| GGGGTCGACGTAAGCAATATAGAGGACAGCAACAAG |
| Cytosolic domain TGN38-5′: |
| TTTGTCGACCACAACAAACGAAAGATTATTGCT |
| Cytosolic domain TGN38-3′: |
| GCCCTCGAGTCAAAGCTTTAGGTTCAAACGTTGGTA |
| TGN38-YQTI-3′: |
| TCAAAGCTTTAGGTTGATAGTTTGGTGTCACT |