Figure 6.

Effects of antibody cross-linking (x-link) and cholesterol depletion on [3H]-oleate uptake. Cells were transiently transfected with FLAG-FAT/CD36 and FATP4 and then treated or not for 30 min with 10 mM methyl-β-cyclodextrin (MβCD). Afterwards overall [3H]-oleate uptake within the first 5 min in presence or absence of anti-FAT/CD36 from Biosource was analysed. Quantification of three independent experiments is shown. The ratio has been arbitrarily set to 100% in cells neither cross-linked nor cholesterol depleted. Cross-linking increased overall fatty acid uptake significantly (p < 0.05). However under conditions of cholesterol depletion, antibody enhanced fatty acid uptake was not apparent.