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. 2008 Aug 18;6:35. doi: 10.1186/1477-7827-6-35

Figure 1.

Figure 1

(A) RT-PCR analysis of ovarian HCN1-4 gene expression. (a) A 248 bp HCN1 RT-PCR band in all three developmental ages studied. (b) A 377 bp HCN2 RT-PCR band was evident in all three developmental ages studied. (c) A 364 bp HCN3 RT-PCR band was expressed in all three developmental stages studied. (d) A 219 HCN4 RT-PCR band was present in all three developmental stages studied. n = 3 animals studied per gene per reproductive stage. M = marker; B = brain; Y = young; A = adult; RA = reproductive aging; N = negative; H = heart. B. Western blot analysis of ovarian lysates for HCN1-4 protein expression of young, adult and reproductively aged rats. (a) Western blot analysis for HCN1 in ovaries. (b) Western blot analysis for HCN2 in ovaries. (c) Western blot analysis for HCN3 in ovaries. (d) Western blot analysis for HCN4 in ovaries. n = 3 animals studied per protein per reproductive stage.