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Immunofluorescence localization of HCN1-4 in young, adult and reproductively aged rat ovaries. Experimental and negative control studies are presented from consecutive ovarian sections for each HCN protein for each reproductive state. (A) Localization of HCN1-4 channels in the young ovary. (B) Localization of HCN1-4 channels in the adult ovary. (C) Localization of HCN1-4 channels in the reproductive aging ovary. n = 3–9 animals studied per protein per reproductive stage.
