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. 2008 Aug 18;6:35. doi: 10.1186/1477-7827-6-35

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Copyright © 2008 Yeh et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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H Score analysis of HCN1-4 channels in the different ovarian structures throughout the aging process. (A) The H scores of HCN1 (a), HCN2 (b), HCN3 (c), and HCN4 (d) channels in the oocytes during the reproductive aging process. (B) H score analysis of granulosa cells in different follicle classes. (a-d) H scores of HCN1-4 channels in primary follicles. (e-h) Results of H score analysis of HCN1-4 channels in preantral follicle granulosa cells. (i-l) H scores for HCN1-4 channels in the granulosa cells of antral follicles. (C) H score analysis of HCN1 (a), HCN2 (b), HCN3 (c), and HCN4 (d) expression in theca cells. (D) H score analysis of HCN1-4 channels (a-d respectively) in the corpora lutea. N/A – not applicable, as the young rats do not yet have corpora lutea. n = 3–9 animals studied per protein per reproductive stage.