Figure 9.
GAIP is distributed near the PM in rat liver. Staining for both GAIP (B) and clathrin (C) is found at the periphery of hepatocytes concentrated along the sinusoidal domain of the PM (arrows). The corresponding phase contrast image is shown in panel A. Semithin cryosections of rat liver were fixed in 2% paraformaldehyde in 100 mM phosphate buffer, pH 7.4, for 1 h, and incubated with affinity-purified, anti-GAIP (C) IgG followed by donkey anti-rabbit F(ab′)2-Texas Red, and examined by immunofluorescence. n, Nucleus. Scale bars, 10 μm.