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. 2008 Sep 26;4(9):e1000166. doi: 10.1371/journal.ppat.1000166

Figure 9. PQS dependent release of extracellular DNA.

Figure 9

PAO1 wild-type, the pqsA, the pqsH and the pqsL mutant were grown in LB medium. Supernatant samples were collected after 8 h (lane 1, 3, 5, 7, 9) and 36 h (lane 2, 4, 6, 8, 10), respectively. Increasing amounts of fragmented DNA were found in the supernatant of the PAO1 wild-type cultures under prolonged incubation (lanes 1, 2). DNA release and fragmentation was delayed in the PAO1 pqsA and pqsH mutants (lane 3, 4 and lane 7, 8). The DNA release and fragmentation pattern was most pronounced in the pqsL mutant (lanes 5, 6) and the complemented pqsA mutant (pqsA-E operon on plasmid pLG10) (lanes 9, 10).