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. 2008 Oct;49(10):2142–2148. doi: 10.1194/jlr.M800082-JLR200

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Copyright © 2008, American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Plasma PON1 activity and liver PON1, apoA-I, and AhR gene expression in LDL receptor (LDLr)^−/−, normal C57BL6, and AhR^−/− mice fed aspirin. A: LDLr^−/−mice (n = 6) were fed an atherogenic diet in the presence and absence of aspirin at a concentration of 2 mg/day for 6 days. Normal C57BL6 mice (n = 6) and AhR^−/−mice (n = 6) were fed an atherogenic diet in the presence and absence of aspirin at a concentration of 2 mg/day for 9 days. Plasma PON1 arylesterase activity was measured using 1 mM ρNPA acetate as substrate, as assessed from the formation of ρNP at 410 nm. PON1 enzyme activity is expressed as percent of control; 100% corresponds to the activity in control mice treated with alcohol alone. B: PON1, apoA-I, and AhR gene expression in liver tissues of LDLr^−/−, normal C57BL6, and AhR^−/− mice fed an atherogenic diet in the presence and absence of aspirin. The filled bars indicate gene expression levels in aspirin-fed mice, and the open bars indicate the control mice that were fed alcohol, the solvent vehicle, alone. C: Induction of AhR gene expression in normal C57BL6 mice fed aspirin as compared with control alcohol-fed mice. RNA extraction and real-time PCR analysis were carried out as described in Materials and Methods. Values are expressed as mean ± SD (n = 3). * P < 0.05; ** P < 0.01.

Fig. 4. — Plasma PON1 activity and liver PON1, apoA-I, and AhR gene expression in LDL receptor (LDLr)^−/−, normal C57BL6, and AhR^−/− mice fed aspirin. A: LDLr^−/−mice (n = 6) were fed an atherogenic diet in the presence and absence of aspirin at a concentration of 2 mg/day for 6 days. Normal C57BL6 mice (n = 6) and AhR^−/−mice (n = 6) were fed an atherogenic diet in the presence and absence of aspirin at a concentration of 2 mg/day for 9 days. Plasma PON1 arylesterase activity was measured using 1 mM ρNPA acetate as substrate, as assessed from the formation of ρNP at 410 nm. PON1 enzyme activity is expressed as percent of control; 100% corresponds to the activity in control mice treated with alcohol alone. B: PON1, apoA-I, and AhR gene expression in liver tissues of LDLr^−/−, normal C57BL6, and AhR^−/− mice fed an atherogenic diet in the presence and absence of aspirin. The filled bars indicate gene expression levels in aspirin-fed mice, and the open bars indicate the control mice that were fed alcohol, the solvent vehicle, alone. C: Induction of AhR gene expression in normal C57BL6 mice fed aspirin as compared with control alcohol-fed mice. RNA extraction and real-time PCR analysis were carried out as described in Materials and Methods. Values are expressed as mean ± SD (n = 3). * P < 0.05; ** P < 0.01.