. 2008 Oct;49(10):2218–2229. doi: 10.1194/jlr.M800297-JLR200

TABLE 1.

Naringenin and insulin similarly stimulate the degradation and inhibit the secretion of apoB-100 from HepG2 cells

	Control	Naringenin	Insulin
LPDS		100 μM	100 nM
Init (arbitrary units)^c	52.23 ± 7.61^a	18.98 ± 2.88^b	25.94 ± 8.18^b
Total degraded (%)^d	93.43 ± 1.25^a	97.67 ± 0.34^b	96.89 ± 0.47^b
Total secreted (%)^e	6.57 ± 1.25^a	2.33 ± 0.34^b	3.11 ± 0.47^b
k(4,3) (pools/min)^f	0.008 ± 0.001^a	0.004 ± 0.001^b	0.004 ± 0.001^b
k(0,3) (pools/min)^f	0.117 ± 0.022^a	0.152 ± 0.014^b	0.122 ± 0.006^a
k(7,3) (pools/min)^f	0.003 ± 0.003^a	0.007 ± 0.003^a	0.010 ± 0.003^a
Portion degraded via rapid pathway (%)^g	91.4 ± 1.6^a	93.9 ± 1.4^a	89.3 ± 2.4^a
Oleate
Init (arbitrary units)^c	86.50 ± 12.8^a	30.08 ± 8.48^b	43.64 ± 9.31^b
Total degraded (%)^d	84.81 ± 4.05^a	96.53 ± 0.87^b	95.66 ± 1.11^b
Total secreted (%)^e	15.19 ± 4.05^a	3.47 ± 0.87^b	4.34 ± 1.11^b
k(4,3) (pools/min)^f	0.008 ± 0.002^a	0.0026 ± 0.0004^b	0.0030 ± 0.0007^b
k(0,3) (pools/min)^f	0.055 ± 0.014^a	0.081 ± 0.010^b	0.070 ± 0.009^a
k(7,3) (pools/min)^f	0.0007 ± 0.0002^a	0.0011 ± 0.0002^a	0.0008 ± 0.0001^a
Portion degraded via rapid pathway (%)^g	83.78 ± 4.04^a	95.29 ± 0.85^b	94.51 ± 1.09^b

Apolipoprotein B-100 (apoB-100) pulse-chase data were analyzed by multicompartmental modeling using SAAM II. The percent of newly synthesized apoB-100 secreted and degraded was determined using the kinetic model. Values are mean ± SEM for lipoprotein-deficient serum (LPDS) (n = 4) and oleate (n = 5).

Means with different letters indicate statistical difference of at least P < 0.05.

The Init parameter is calculated by the model and represents the amount of radioactive apoB-100 entering the system (initially compartment 3) required to achieve the tracer curve for total apoB (cell plus media) measured experimentally.

Calculated using the formula [k(0,3) + k(7,3) / k(4,3) + k(0,3) + k(7,3)] × 100.

Calculated using the formula [k(4,3) / k(4,3) + k(0,3) + k(7,3)] × 100.

k(4,3) is the rate constant for apoB-100 transfer from compartment 3 to compartment 4, or the rate constant for secretion. k(0,3) is the rate constant for apoB-100 degradation directly from compartment 3, or the rate constant for rapid degradation. k(7,3) is the rate constant for apoB-100 transfer from compartment 3 to compartment 7, or the rate constant for slow degradation.

The percent of apoB-100 degraded directly from compartment 3 is calculated using the formula [k(0,3) / k(4,3) + k(0,3) + k(7,3)] × 100.