TABLE 2.
Ca2+ registrations in SW620 cells pretreated with DHA (70 μM) at time points indicated: SW620 cells stimulated with ATP, 1 μM
| Pretreatment | # Cells (Responding Cells, %) | Maximal [Ca2+] Increase, nM | Decline of the Response |
|---|---|---|---|
| None (control) | 120 (56) | 190 (± 13.2) | 1.64 (± 0.06) |
| DHA 6 h | 110 (53) | 196 (± 10.8) | 1.66 (± 0.16) |
| DHA 12 h | 107 (55) | 226 (± 15.9)a | 1.39 (± 0.06)a |
| DHA 24 h | 94 (51) | 201 (± 18.4) | 1.27 (± 0.09)b |
| DHA 48 h | 97 (45) | 198 (± 21.1) | 1.23 (± 0.04)b |
After 30 s of [Ca2+] registration ATP (1μM) (Sigma-Aldrich) or vehicle was added. Maximal [Ca2+] increase is calculated as difference between baseline and peak [Ca2+] in the responding cells. The decline of the response is quantified as the ratio between peak [Ca2+] and [Ca2+] at the end of the registration (180 s). Registrations are depicted in Fig. 4C. The data are presented as means with standard errors (± SEM).
a
Statistically significant difference from control (P < 0.05).
b
Statistically significant difference from control (P < 0.01).