. 2008 Oct;49(10):2089–2100. doi: 10.1194/jlr.M700389-JLR200

TABLE 3.

Ca²⁺ registrations in SW620 cells pretreated with DHA (70 μM) at time points indicated: SW620 cells stimulated with ATP, 1μM

Pretreatment	# Cells	Baseline [Ca²⁺], nM	Maximal [Ca²⁺] Increase, nM	Decline of the Response
None (control)	103	123 (± 4.1)	247 (± 10.6)	2.07 (± 0.09)
DHA 24 h	85	141 (± 4.7)^a	238 (± 14.1)	1.61 (± 0.07)^a
2. EGTA	81	103 (± 3.7)^a	259 (± 18.2)	2.51 (± 0.14)^a
EGTA, DHA 24 h	73	105 (± 3.8)^a^,^b	227 (± 17.3)	2.11 (± 0.12)^a^,^b

EGTA, ethylene glycol tetraacetic acid. After 30 s of [Ca²⁺] registration ATP (1μM) (Sigma-Aldrich) or vehicle was added. Maximal [Ca²⁺] increase is calculated as difference between baseline and peak [Ca²⁺] in the responding cells. The decline of the response is quantified as the ratio between peak [Ca²⁺] and [Ca²⁺] at the end of the registration (180 s). Registrations are depicted in Fig. 4D. The data are presented as means with standard errors (± SEM).

Significantly different from control (P < 0.05).

Significantly different from DHA 24 h (÷ EGTA) (P < 0.001).