Fig. 3.

Desaturation indices of oleate-stearate (18:1 n-9/18:0) and palmitoleate-palmitate (16:1/16:0) in [U-¹³C]stearate (A, C) and [U-¹³C]palmitate (B, D) experiments. Each bar represents the average and SEM of triplicate cell culture experiments. * P ⩽ 0.001, ** P ⩽ 0.003. In the experiment with [U-¹³C]stearate, the average oleate-stearate ratio was significantly decreased (P < 0.001). In the experiment with [U-¹³C]palmitate, the average oleate-stearate ratio was mildly decreased, but not significantly in the CGX0168 (P = 0.14) and CGX290 groups (P = 0.25); it approached significance in the CLA group (P = 0.08). Both experiments showed a significant decrease in the palmitoleate/palmitate desaturation indices with the inhibitors. With [U-¹³C]stearate, the average palmitoleate-palmitate ratio was significantly decreased in the CGX0168 and CGX0290 groups (P < 0.001), but it was increased in the CLA group (P = 0.0029). With [U-¹³C]palmitate, the average ratio was significantly decreased for both the CGX0168 group (P = 0.003) and the CGX0290 group (P < 0.001). However, in the CLA group, the difference was not significant, with a trend toward an increased ratio (P = 0.13).