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. Author manuscript; available in PMC: 2008 Sep 11.
Published in final edited form as: J Leukoc Biol. 2006 Nov 9;81(2):520–527. doi: 10.1189/jlb.0406262

Fig. 4.

Fig. 4

Effects of high glucose concentration and hyperosmolarity on O2- generation from normal human mononuclear phagocytes, which were isolated from healthy controls (n=4) and cultured in RPMI 1640 with low glucose (5.5 mM D-glucose), high glucose (25.5 mM D-glucose), mannitol (5 mM D-glucose, 20 mM mannitol), or L-glucose (5.5 mM D-glucose, 20 mM L-glucose). The cells were assayed after 2 days for O2- anion generation in the resting state or under stimulation with PMA or OPZ. The amount of O2- production was adjusted by total protein content of each well (nmol O2-/mg total protein). All values represent the average ± se (*, P<0.05, compared with low glucose; #, P<0.01, compared with low glucose).