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. Author manuscript; available in PMC: 2008 Sep 11.
Published in final edited form as: J Leukoc Biol. 2006 Nov 9;81(2):520–527. doi: 10.1189/jlb.0406262

Fig. 7.

Fig. 7

Expression of RAGE on THP-1 cells and human mononuclear phagocytes. THP-1 cells were treated with normal culture medium (RPMI 1640 with 25 mM D-glucose) or high glucose medium (RPMI 1640 with 50 mM D-glucose) for 2 days before being lysed for Western blotting for RAGE expression at protein level. Human mononuclear phagocytes were isolated as described. With or without prior mixing with blocking peptide (1:50), RAGE N-16 antibody (1:200) was used to detect the presence of the full-length RAGE protein (50 kDa). Results are representative of three independent experiments.