Table 2.
Comparison of the relative abundances of the identified compounds of rat preputial glands between groups (mean ± standard deviation, N = 5 for each group)
| GC peak No. | Retention time (min) | Compound identification |
Experimental groups |
||||
| Compound names | Diagnostic ions [m/z(relative intensity)] | Males | Females | Castrated males | Ovariectomized females | ||
| 1 | 4.82 | Benzaldehyde | 106(100), 77(88), 105(86), 51(46) | 0.16 ± 0.11 | 0.21 ± 0.12c | 0.24 ± 0.25 | 0.09 ± 0.02c |
| 2 | 9.78 | Unkown | 59(100), 41(70), 56(66), 127(65), 43(53), 69(26), 84(18), 142(4) | 0.02 ± 0.003a,b | 0.21 ± 0.09a,c | 0.07 ± 0.02b | 0.11 ± 0.06c |
| 3 | 12.17 | 3,7-Dimethyl-2,6-octadienal | 41(100), 69(99), 84(34), 39(24), 94(15), 152(6) | 0.05 ± 0.02 | 0.05 ± 0.02 | 0.05 ± 0.02 | 0.05 ± 0.02 |
| 4 | 12.71 | Indole* | 117(100), 90(34), 89(26), 118(10), 63(8) | 0.08 ± 0.06 | 0.10 ± 0.08 | 0.10 ± 0.06 | 0.04 ± 0.01 |
| 5 | 15.17 | E-ß-farnesene* | 69(100), 41(86), 93(60), 81(24), 133(24), 120(22), 204(2) | 0.03 ± 0.01a,b | 0.13 ± 0.06a | 0.11 ± 0.04b | 0.12 ± 0.03 |
| 6 | 15.89 | E,E-α-farnesene* | 93(100), 41(74), 55(54), 69(50), 107(48), 119(46), 123(45), 91(38), 79(36), 204(1) | 0.08 ± 0.03a,b | 0.32 ± 0.14a | 0.28 ± 0.09b | 0.31 ± 0.09 |
| 7 | 16.71 | Dodecanoic acid | 60(100), 73(98), 43(78), 129(46), 157(28), 171(8), 200(6) | 0.47 ± 0.33a,b | 0.10 ± 0.09a | 0.04 ± 0.05b | 0.07 ± 0.03 |
| 8 | 18.98 | Tetradecanoic acid* | 73(100), 60(96), 43(86), 41(70), 57(76), 55(68), 129(66), 185(26), 228(12) | 0.82 ± 0.38a,b | 0.30 ± 0.10a | 0.28 ± 0.13b | 0.25 ± 0.10 |
| 9 | 19.49 | Hexadecanal | 57(100), 43(96), 68(46), 71(35), 82(86), 96(56), 110(18), 124(12), 138(6), 222(1) | 0.23 ± 0.17b | 0.47 ± 0.47 | 0.63 ± 0.37b | 0.12 ± 0.11 |
| 10 | 21.09 | Hexadecanoic acid* | 43(100), 73(94), 60(90), 129(52), 213(12), 256(14) | 7.61 ± 2.79 | 5.59 ± 1.72 | 8.27 ± 2.45 | 7.02 ± 2.07 |
| 11 | 22.69 | Z9,Z12-octadecenoic acid* | 67(100), 81(84), 55(82), 41(72), 95(64), 109(30), 60(10), 280(6) | 2.39 ± 0.96 | 2.30 ± 0.90 | 2.15 ± 0.79 | 2.47 ± 0.78 |
| 12 | 22.73 | Z9-octadecenoic acid* | 55(100), 69(80), 83(86), 97(82), 43(64), 60(16), 264(12), 282(1) | 1.87 ± 1.00 | 1.99 ± 0.85 | 2.20 ± 1.05 | 2.38 ± 1.23 |
| 13 | 22.76 | E9-octadecenoic acid* | 55(100), 41(66), 43(48), 69(56), 83(46), 97(42), 111(18), 60(12), 264(2), 282(1) | 1.42 ± 0.72a | 0.80 ± 0.05a | 1.35 ± 0.76 | 0.75 ± 0.22 |
| 14 | 22.95 | Octadecanoic acid* | 43(100), 57(80), 73(80), 60(74), 55(72), 129(56), 84(22), 185(20), 241(12) | 4.31 ± 1.58a | 2.72 ± 0.74a | 5.53 ± 2.41 | 2.97 ± 0.89 |
| 15 | 24.15 | (all-Z)5,8,11,14-eicosatetraenoic acid | 79(100), 41(86), 67(76), 91(70), 80(72), 55(70), 105(38), 119(30), 304(0.5) | 0.90 ± 0.43a | 0.44 ± 0.17a,c | 0.89 ± 0.66 | 0.84 ± 0.35c |
| 16 | 24.26 | (all-Z)8,11,14-eicosatrienoic acid | 67(100), 79(96), 41(92), 80(88), 55(86), 93(54), 94(42), 150(22), 60(14), 306(1) | 0.38 ± 0.22a | 0.13 ± 0.06a | 0.29 ± 0.19 | 0.17 ± 0.09 |
| 17 | 24.41 | Z11,Z14-eicosadienoic acid | 67(100), 55(88), 81(84), 41(74), 95(66), 109(32), 60(14), 123(12), 308(4) | 2.45 ± 0.64 | 2.06 ± 1.20 | 2.81 ± 1.64 | 1.38 ± 0.45 |
| 18 | 25.77 | A tricosadienoic acid | 79(100), 41(82), 67(82), 55(74), 80(80), 91(66), 93(56), 105(34), 119(26), 133(12), 150(12), 60(10), 340(0.5) | 0.83 ± 0.42 | 0.62 ± 0.33 | 1.66 ± 1.31 | 0.81 ± 0.41 |
| 19 | 26.03 | A pentanoic acid ester | 103(100), 55(34), 81(26), 57(24), 67(20), 69(20), 96(18), 95(16), 97(10), 41(18), 43(16), 85(4) | 0.14 ± 0.05 | 0.11 ± 0.02 | 0.23 ± 0.11 | 0.10 ± 0.03 |
| 20 | 26.18 | A pentanoic acid ester | 103(100), 57(26), 43(12), 41(8), 55(44), 81(2), 83(2), 85(1) | 0.13 ± 0.05b | 0.14 ± 0.09 | 0.29 ± 0.14b | 0.13 ± 0.04 |
| 21 | 28.17 | Squalene* | 69(100), 81(56), 41(26), 95(16), 93(14), 55(8), 107(8), 121(12), 123(10), 136(10), 137(10), 109(9) | 46.67 ± 7.22b | 50.49 ± 6.00 | 35.24 ± 10.77b | 50.13 ± 5.71 |
| 22 | 28.79 | A terpenoid polyene | 69(100), 93(44), 41(42), 135(40), 81(32), 55(20), 43(18), 107(18), 148(14), 147(12), 175(4), 203(2) | 3.15 ± 0.50 | 3.37 ± 1.18 | 3.07 ± 0.59 | 2.35 ± 0.55 |
| 23 | 29.04 | A terpenoid polyene | 69(100), 81(100), 43(68), 41(50), 71(49), 95(34), 123(30), 109(26), 93(25), 107(22), 121(20), 135(20), 136(16), 203(2) | 0.35 ± 0.19b | 1.97 ± 2.75 | 0.65 ± 0.26b | 0.27 ± 0.29 |
| 24 | 29.17 | A terpenoid polyene | 69(100), 151(66), 109(56), 41(50), 123(34), 81(28), 93(12), 95(12), 203(2) | 1.51 ± 0.31b | 1.11 ± 0.38 | 1.10 ± 0.29b | 0.90 ± 0.28 |
| 25 | 29.44 | A terpenoid polyene | 69(100), 151(82), 41(32), 123(20), 81(10), 109(4), 93(2), 95(2) | 2.26 ± 0.41b | 2.35 ± 0.27 | 1.31 ± 0.82b | 1.91 ± 0.79 |
| 26 | 29.55 | A terpenoid polyene | 69(100), 95(38), 41(36), 81(28), 59(20), 153(14), 109(12), 107(10), 123(10), 121(8) | 5.10 ± 0.68a,b | 4.30 ± 0.33a | 2.44 ± 1.27b | 3.38 ± 1.18 |
| 27 | 29.76 | A terpenoid polyene | 69(100), 41(36), 81(26), 203(24), 119(20), 123(16), 93(12), 95(8) | 4.89 ± 0.68 | 5.31 ± 1.51 | 5.40 ± 0.94 | 4.51 ± 1.04 |
| 28 | 30.54 | A terpenoid polyene | 43(100), 69(78), 71(78), 121(66), 41(54), 95(52), 81(50), 59(52), 125(48), 139(16), 149(10), 167(4) | 1.29 ± 0.18 | 1.64 ± 0.54c | 0.91 ± 0.36 | 0.96 ± 0.31c |
| 29 | 30.90 | Cholesterol* | 43(100), 55(78), 107(72), 57(70), 105(68), 145(66), 95(64), 386(52), 91(50), 275(38), 213(26), 301(32), 369(20) | 10.34 ± 1.26b | 10.28 ± 2.36c | 22.02 ± 4.86b | 15.07 ± 2.32c |
The means in a row marked by same superscript letters show significant differences (P < 0.05, using independent t-test or Mann–Whitney U test). The compounds marked by superscript asterisk (*) were verified with authentic standards; other components were identified by comparison with spectra listed in the NIST (Agilent Technologies 2002) mass spectral library and analogous data.