Table 1.

Stimuli used for CT experiments

Rinse	M	Salt stimuli	M
R	0.01 KCl	N	0.01 KCl + 0.1 NaCl
R	0.01 KCl	N + Bz	0.01 KCl + 0.1 NaCl + 5 × 10−6 Bz
R	0.01 KCl	N + Bz + MRPs	0.01 KCl + 0.1 NaCl + 5 × 10−6 Bz + MRPs (0–1%)
R	0.01 KCl	N + Bz + MRPs + TRPV1t modulatorsa	0.01 KCl + 0.1 NaCl + 5 × 10−6 Bz + MRPs (0–1%) + TRPV1t modulatorsa
R	0.01 KCl	MSG + Bz + SB	0.01 KCl + 0.1 MSG + 5 × 10−6 Bz + 1 × 10−6 Bz SB
R	0.01 KCl	MSG + Bz + SB + IMP	0.01 KCl + 0.1 MSG + 5 × 10−6 Bz + 1 × 10−6 Bz SB + 1 × 10−3 IMP
(R)pHb	0.01 KCl + 0.01 HEPES/Tris	(N + Bz + MRPs)pHb	0.01 KCl + 0.01 HEPES/Tris + 0.1 NaCl + 5 × 10−6 Bz + MRPs (0–1%)
R	0.01 KCl	Control solution 1	0.3 NH4Cl
R	0.01 KCl	Control solution 2	0.3 NaCl

MRPs: GalA-MRP; GlcNH₂-MRP; Xyl-MRP; Fru-MRP; Glc-MRP. The concentration of MRPs is in percent. In some experiments, we also tested the effect of the unreacted soy protein fraction.

^aTRPV1 modulators: RTX (0.25 × 10⁻⁶ M); CAP (20 × 10⁻⁶ M); ethanol (20% or 40%); SB (N-(3-methoxyphenyl)-4-chlorocinnamide; SB-366791), a specific blocker of TRPV1t. In some experiments, SB was used at 5 × 10⁻⁶ M; Bz, a specific blocker of the epithelial Na⁺ channel.

^bHEPES (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid) or Tris (tris(hydroxymethyl)aminomethane) were used to buffer the pH of (R)_pH and (N + Bz + MRPs)_pH between pH 4.1 and 9.1.