Table 2.
Oxidative properties of subsarcolemmal mitochondria (SSM) and interfibrillar mitochondria (IFM) isolated from heart of control and heart failure dogs
| Control | Heart failure | |
|---|---|---|
| SSM | ||
| State 3 | 514 ± 41 | 214 ± 33* |
| State 4 | 15 ± 5 | 15 ± 3 |
| RCR | 34 ± 7 | 18 ± 4 |
| ADP/O | 2.77 ± 0.09 | 2.69 ± 0.02 |
| Maximal ADP | 619 ± 41 | 191 ± 22* |
| DNP | 608 ± 32 | 191 ± 38* |
| IFM | ||
| State 3 | 472 ± 26 | 223 ± 12* |
| State 4 | 20 ± 6 | 16 ± 4 |
| RCR | 39 ± 14 | 17 ± 3 |
| ADP/O | 2.78 ± 0.15 | 2.85 ± 0.15 |
| Maximal ADP | 529 ± 16 | 221 ± 17* |
| DNP | 535 ± 10 | 206 ± 19* |
Glutamate as substrate. RCR, respiratory control ratio (state 3/state 4); ADP/O, ADP to atomic oxygen phosphorylation ratio; Maximal ADP, state 3 with saturating 2 mM ADP; DNP, dinitrophenol. Respiratory rates are expressed as nA O/min/mg mitochondrial protein. n= 5 for control and n = 5 for HF. Mean ± SEM.
*P < 0.05.