. Author manuscript; available in PMC: 2008 Sep 11.

Published in final edited form as: Biochemistry. 2006 Sep 26;45(38):11401–11413. doi: 10.1021/bi060691x

Table 2.

Thermal denaturation of ferric wild-type and A69S rHb-R

Protein	T_M (°C)^a	ΔH° (kJ mol⁻¹)^b	ΔC_p (kJ mol⁻¹ K⁻¹)
wt (vis and CD)	73.6 ± 0.2	288 ± 10	7.1^c
A69S (vis)	71.15 ± 0.04	284 ± 3	7.1^c
A69S (CD)	69.62 ± 0.13	271 ± 6	7.1^c
CN-A69S (vis)	73.99 ± 0.05	368 ± 4	5.8 ± 1.8

Midpoint of thermal denaturation in pH 7.2, 20 mM phosphate buffer. Errors reported are standard deviations from global fitting of multiple data sets.

Change in enthalpy evaluated at the midpoint of the thermal unfolding transition.

Value fixed during fitting.