. Author manuscript; available in PMC: 2008 Sep 11.

Published in final edited form as: Biochemistry. 2006 Sep 26;45(38):11401–11413. doi: 10.1021/bi060691x

Table 3.

Urea denaturation of ferric wild-type and A69S rHb-R

	ΔG^o (kJ mol⁻¹) ^a	m (kJ mol⁻¹ M⁻¹) ^b	C_m (M) ^c
wt (vis)	30.6 ± 0.3	5.99 ± 0.05	5.11
wt (CD)	27.4 ± 0.3	5.57 ± 0.06	4.9
A69S (vis)	28.38 ± 0.17	5.94 ± 0.04	4.78
A69S (CD)	28.6 ± 0.4	6.25 ± 0.08	4.6

Free energy of unfolding at 25 °C in pH 7.2, 20 mM phosphate buffer. Errors reported are standard deviations from global fitting of multiple data sets.

Dependence of free energy of unfolding on denaturant concentration.

Midpoint of urea denaturation, calculated from ΔG^o and m.