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Effects of GSH addition on the yields of the sulfinic acid (M1) and carbodiimide (M2) metabolites of TAZ: (Top) Addition of increasing amounts of GSH to incubations of TAZ, NADPH and EtaA resulted in a concentration-dependent reduction in the yields of both M1 and M2; (Bottom) When GSH was added after the incubations were complete, only M2 reacted with GSH to form an adduct. GSH-M2 adduct formation was observed after adding 0.25 mM or more GSH.
