TABLE 5.
IC50 and CC50 values of moxifloxacin, ciprofloxacin, and NXL101 against the gyrase supercoiling reaction and against cleaved-complex stabilization of WT and mutant S. aureus DNA gyrases
| Antibiotic | IC50 or CC50 (μM) against gyrasea:
|
|||||||
|---|---|---|---|---|---|---|---|---|
| WT
|
Mutant
|
|||||||
| S84L E88K
|
M121K
|
D83N
|
||||||
| IC50 | CC50 | IC50 | CC50 | IC50 | CC50 | IC50 | CC50 | |
| Ciprofloxacin | 27 | 11.2 | >200 | >200 | 94 | 24 | >200 | 50 |
| Moxifloxacin | 17 | 2.5 | >200 | >200 | 58 | 5 | 44 | 19 |
| NXL101 | 1.0 | >200 | 0.8 | >200 | >200 | >200 | >200 | >200 |
a
Shown are IC50 and CC50 values of moxifloxacin, ciprofloxacin, and NXL101 against the gyrase supercoiling reaction (IC50) and against cleaved-complex stabilization (CC50) of WT and mutant forms of S. aureus DNA gyrase.