TABLE 2.
Activities of HCV-796 against replicon variants
| Cellsa | EC50 (μM) ± SEb | Reduced susceptibility (n-fold) | Viral load (copies/μg total RNA) ± SEc | Mean log10 ± SE viral reductiond |
|---|---|---|---|---|
| Clone A | 0.013 ± 0.013 (n = 8) | 3.7 × 108 ± 2.4 × 108 | 2.1 ± 0.4 (0.7) | |
| 796R(0.1 μM) | 0.3 ± 0.2 (n = 4) | 23 | 2.9 × 108 ± 0.5 × 108 | 1.4 ± 0.3 (56) |
| 796R(1 μM) | 8.0 ± 5.2 (n = 12) | 618 | 2.4 × 108 ± 2.0 × 108 | 0.7 ± 0.2 (56) |
| 796R(10 μM) | >56.0 (n = 4) | >6,812 | 4.1 × 108 ± 1.7 × 108 | 0.5 ± 0.3 (56) |
a
796R represents cells that are less susceptible to HCV-796.
b
EC50 values were determined using MDL LSW data analysis. Inhibitory activity is expressed as mean EC50 ± standard error. n indicates the number of independent experiments.
c
Steady-state levels of HCV RNA after 3 days of incubation in tissue culture medium. The results represent at least three independent determinations.
d
Viral load reduction was determined at the indicated compound concentrations (μM, in parentheses) in a 3-day assay. The results represent at least three independent determinations.