Phylogenetic locations of the study isolates. Study isolates were placed on a previously created phylogenetic tree (which had been based on an analysis of 212 SNPs in a global collection of 327 M. tuberculosis isolates). The SCGs, including subgroups, and the positions of the M. tuberculosis reference strains 210, CDC1551, and H37Rv and of M. bovis strain AF2122/97 are indicated. The total number of distinct strains, as defined by DNA fingerprinting, within each SCG is given in parentheses.