TABLE 2.
Primers and dually labeled probes developed in this study
| Primer/probe | Sequence (5′-3′)a |
|---|---|
| ACT1F | CGCTTTGGACTTCGAACAAGAA |
| ACT1R | GTTACCGATGGTGATGACTTGAC |
| ACT1 probe | Cy5-AACCGCTGCTCAATCTTCCTCCAT-BHQ-2 |
| PDH1F | CAGACCCGGTTCCGGTTGTA |
| PDH1R | CGGTAGTGCTTCTTGATCTCGTT |
| PDH1 probe | FAM-ACGCTGCTGAAGTCCATCTCCTCG-TAMSp |
| CDR1F | TTAAAAGTTCAAGCCAGTATTTCC |
| CDR1R | AAATTTGATAACCATCGTAAAGCA |
| CDR1 probe | FAM-ACGCTGCTGCTACTGTGGCTATCT-TAMSp |
| PDR1F | TACATGGAACATCTGTTGCTTCTT |
| PDR1R | CGACTCTTCATAGCCGACGT |
| PDR1 probe | FAM-TGAAGAACAGCTTGCTCTCGACGA-TAMSp |
a
FAM, 6-carboxyfluorescein; BHQ-2, black hole quencher 2; TAMSp, 6-carboxytetramethylrhodamine.