FIG. 1.

CPP activity against asynchronous blood-stage P. falciparum cultures. (A-C) Following 24-h incubation with 30 μM pVEC or TP10 at 2% hematocrit, antiparasitic activity was measured using the lactate dehydrogenase assay (A). Background absorbance obtained with uninfected RBCs was subtracted from the other readings. Bars with different letters are significantly different (one-way analysis of variance; P < 0.0001). (B) The time course of TP10 activity was monitored every 3 h over the 24-h incubation period and compared to control cultures (i.e., phosphate-buffered saline [PBS] added instead of a CPP) to determine the time course of its antimalarial activity. *, indicates a significant difference (P < 0.05) using Student's t test (pairwise comparison). (C) The hemolytic activity of TP10 against human RBCs was analyzed over three different concentrations (1, 10, and 30 μM) and compared to a Triton X-100 positive control, where lysis was assumed to be 100%. Error bars in all figures represent the standard errors of the means (n = 3).