TABLE 1.
DNA sequences used in the target gene amplification studies and real-time RT-PCR experiments
| Study type and primer or probe | Primer or probe sequence (5′-3′)a |
|---|---|
| Target gene amplification studies | |
| ribofor | GGACAACATCTCGAAAGGGA |
| riborev | GCGATTACTAGCGATTCCGA |
| ompAfor | GGCTTGAGCTTGAACAACAA |
| ompArev | TGTTCAGCTAAAACAGTACGGC |
| adeBfor | CGGAAGGCATGGAGTTTAGT |
| adeBrev | CTGCCATTGCCATAAGTTCA |
| abeMfor | TGCAACGCAGTTTCATTTTT |
| abeMrev | CGATGTTTCATCGGCTTTTT |
| adeRfor | AGCGTATGATGAGTTGAAGCA |
| adeRrev | AATCCAGCCTTTTTCAATCG |
| adeSfor | CGTGGCGTGGGATATAGACT |
| adeSrev | AGGAAAATGCCACAAAATGG |
| adeS2for | TCAAATGTTAATTAATGTGCGTGG |
| adeS2rev | TTGTTGTTTGGCATAAAGAGTTGT |
| Real-time RT-PCR studies | |
| riboFor | GTAGCGGTGAAATGCGTAGA |
| riboRev | CTTTCGTACCTCAGCGTCAG |
| riboProbe | [DFAM]CGAAGGCAGCCATCTGGCCT[DTAM] |
| ampCFor | TGCTATTTCAAAGGAACCTTCA |
| ampCRev | TTAATGCGCTCTTCATTTGG |
| ampCProbe | [DFAM]TGGCTCAACTAACGGTTTCGGAAC[DTAM] |
| ompAFor | AGCTCTTGCTGGCTTAAACG |
| ompARev | GAGCAACTGGAGTTGGTTCA |
| ompAProbe | [DFAM]CAGCAGGCTTCAAGTGACCACCA[DTAM] |
| adeBFor | TACGCTTATTCCAGCGATTG |
| adeBRev | CCGAACATGGTGAGTACGTT |
| adeBProbe | [DFAM]AGCCGGCAAGCAACATCACG[DTAM] |
| adeMFor | GCTATTCCGAAGCATTAGGC |
| adeMRev | CCAAAGCAGGTATTGGTCCT |
| adeMProbe | [DFAM]CCCGCCCTGTCACGGTCATT[DTAM] |
a
DFAM, 6-carboxyfluorescein; DTAM, 6-carboxytetramethylrhodamine.