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. Author manuscript; available in PMC: 2008 Sep 11.
Published in final edited form as: Biochemistry. 2007 Jun 13;46(27):7942–7952. doi: 10.1021/bi700543x

Figure 2.

Figure 2

pH-rate profiles for the hydrolysis of substrates by NagA (A) log kcat vs. pH profiles for Zn-NagA with N-acetyl-d-glucosamine-6-phosphate ( ○ ) and N-acetyl-d-glucosamine-6-sulfate ( ● ). The solid lines represent fits of the data to equation 3. (B) logk cat/Km vs. pH profiles for Zn-NagA with N-acetyl-d-glucosamine-6-phosphate ( ○ ) and N-acetyl-d-glucosamine-6-sulfate ( ● ). The solid lines represent fits of the data to equations 5 and 3, respectively. Additional details are provided in the text and the dissociation constants from fits of the data to the appropriate equations are listed in Table 3.