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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: FEBS Lett. 2008 Aug 4;582(20):3033–3036. doi: 10.1016/j.febslet.2008.07.042

Figure 1.

Figure 1

Nitrotyrosine formation of the calcium channel by peroxinitrite. Nitration of Cav1.2b transfected HEK293 cells and GST-CT proteins were generated by ONOO− as described in “Materials and Methods”. Western blots demonstrating the effect of increasing ONOO− concentration on a) Cav1.2b (top panel) calcium channel and b) CT fragments (bottom right). Samples were co-immunoblotted with anti-nitrotyrosine (anti-NY) and calcium channel antibodies (anti-Cav1.2 or anti-CT). IR-conjugated secondary antibodies were used : anti-NY (green) and anti-calcium channel (red). Merged images in top panels show nitration of the calcium channel.