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. Author manuscript; available in PMC: 2009 Sep 3.
Published in final edited form as: FEBS Lett. 2008 Aug 4;582(20):3033–3036. doi: 10.1016/j.febslet.2008.07.042

Figure 4.

Figure 4

Denitration restores src kinase induced tyrosine phosphorylation. GST-CT proteins were nitrated by ONOO− and either treated with cell lysates from LPS-activated RAW264.7 cells or not prior to phosphorylation by c-src kinase (9 units/mL) incubation. Immunoblots with anti-phosphotyrosine (anti-PY) antibody showed phosphorylation of nitrated GST-CT after treatment of RAW264.7 cell lysates whereas nitration of GST-CT prevented phosphorylation by c-src kinase (right panel). Bottom graph shows relative amount of signal intensity for phosphorylation (n=3)