Table 1.
Sample status | Sample name | Sex | Age | Total reads | Total read lengtha (bp) | contigs |
singlets |
Total lengh of assembled sequence (Mb) | Total genesb (A) | COG-assigned genes (B) | Identified COGsc (C) | Average COG sized (B)/(C) | In-house NR DB hit genese (D) | Orphan genesf (A) − (D) | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
average length (bp) | contigs | average length (bp) | reads | |||||||||||||
Individual | In-A | Male | 45 years | 81687 | 52509363 | 2809.0 | 5410 | 902.0 | 16330 | 29.93 | 38778 | 18058 | 2355 | 7.67 | 30210 | 8568 |
Individual | In-B | Male | 6 months | 80617 | 62792581 | 3701.6 | 1721 | 1003.1 | 8481 | 14.88 | 20063 | 9393 | 1617 | 5.81 | 15127 | 4936 |
Individual | In-D | Male | 35 years | 84237 | 55137918 | 2159.1 | 7613 | 911.8 | 36312 | 49.55 | 67740 | 29998 | 2559 | 11.72 | 49079 | 18661 |
Individual | In-E | Male | 3 months | 80852 | 56781600 | 2301.1 | 4819 | 1008.7 | 16838 | 28.07 | 37652 | 18688 | 2107 | 8.87 | 28513 | 9139 |
Individual | In-M | Female | 4 months | 89340 | 57808421 | 3375.1 | 4794 | 655.7 | 15541 | 26.37 | 34330 | 18187 | 2857 | 6.37 | 27050 | 7280 |
Individual | In-R | Female | 24 years | 85787 | 55404826 | 1920.6 | 8935 | 811.3 | 36524 | 46.79 | 63356 | 28612 | 2655 | 10.78 | 46104 | 17252 |
Family I | F1-S | Male | 30 years | 78452 | 53568019 | 2153.6 | 7545 | 806.5 | 28038 | 38.86 | 54151 | 25173 | 2531 | 9.95 | 40771 | 13380 |
F1-T | Female | 28 years | 81348 | 55365235 | 1980.4 | 7389 | 791.5 | 37458 | 44.28 | 65156 | 31230 | 2921 | 10.69 | 47955 | 17201 | |
F1-U | Female | 7 months | 82525 | 53864663 | 2779.4 | 4854 | 850.4 | 14430 | 25.76 | 35260 | 20365 | 2519 | 8.08 | 28711 | 6549 | |
Family II | F2-V | Male | 37 years | 80772 | 55926002 | 2008.7 | 7919 | 809.2 | 38442 | 47.02 | 66461 | 33535 | 2873 | 11.67 | 49955 | 16506 |
F2-W | Female | 36 years | 79163 | 54885684 | 2289.8 | 6778 | 833.0 | 30550 | 40.97 | 57213 | 27680 | 2609 | 10.61 | 43625 | 13588 | |
F2-X | Male | 3 years | 80858 | 56587120 | 2296.0 | 5032 | 832.1 | 34252 | 40.05 | 57446 | 26599 | 2669 | 9.97 | 42452 | 14994 | |
F2-Y | Female | 1.5 years | 79754 | 56276047 | 2044.2 | 9159 | 849.9 | 32461 | 46.31 | 64942 | 30870 | 2664 | 11.59 | 50349 | 14593 | |
Total | 1065392 | 726907479 | 2299.8 | 81968 | 839.9 | 345657 | 478.84 | 662548 | 318388 | 3268 | 97.43 | 499901 | 162647 | |||
American | Sub. 7 | Female | 28 years | 15.94 | 22329 | 12223 | 2160 | 5.66 | 18443 | 3886 | ||||||
Sub. 8 | Male | 37 years | 20.49 | 27579 | 14962 | 2249 | 6.65 | 23518 | 4061 | |||||||
Soil | 144.90 | 212128 | 84060 | 4423 | 19.01 | 125302 | 86826 | |||||||||
Whale fall | 1 | 29.78 | 46478 | 23222 | 3140 | 7.40 | 32847 | 13631 | ||||||||
2 | 30.86 | 47274 | 21826 | 2922 | 7.47 | 32038 | 15236 | |||||||||
3 | 28.87 | 42870 | 21076 | 2894 | 7.28 | 31410 | 11460 | |||||||||
Sargasso | 816.55 | 1406274 | 746941 | 5184 | 144.09 | 1038259 | 368015 |
aThe total read length with Phred scores of >15.
bThe number of genes predicted from the non-redundant sequences of each sample by the MetaGene program.
cThe number of COGs to which at least one gene was assigned in each microbime.
dThe average numbers of predicted genes belonging to each COG [(B)/(C)].
eThe number of predicted genes showing similarity to genes in the ‘in-house extended NR database’.
fThe number of genes showing no significant similarity to genes in the ‘in-house extended NR database’ (e-value ≥ 1.0e − 5 in the BLASTP analysis).