Table 2.

Amino Acid Replacement Matrix (AARM) for medium-GC group in human mouse orthologs

		Human
		Gly	Ala	Arg	Pro	Phe	Tyr	Met	Ile	Asn	Lys	Ser	Thr	Cys	Trp	Val	Leu	Glu	Asp	His	Gln
Mouse	Gly	–	0.92	0.90	1.09	0.93	0.9	1.04	0.76	0.84	0.83	0.92	0.73	0.89	0.90	1.10	1.20	0.97	0.88	0.69	0.80
	Ala	1.09	–	1.19	1.08	1.19	1.00	0.9	0.85	0.97	1.05	0.85	0.85	0.8	0.59	0.95	0.96	0.90	0.74	1.06	1.21
	Arg	1.11	0.84	–	1.02	0.91	0.84	0.85	0.76	1.04	1.09	1.10	1.07	1.07	1.23	0.91	0.92	1.12	0.98	0.95	0.89
	Pro	0.92	0.93	0.98	–	0.76	0.61	1.21	0.82	0.79	1.03	0.82	0.97	0.62	1.75	0.77	0.85	1.05	0.78	1.11	1.15
	Phe	1.08	0.84	1.10	1.32	–	1.19	0.72	0.95	1.41	1.67	0.98	0.93	0.97	1.38	0.82	1.01	1.80	0.93	1.62	1.15
	Tyr	1.11	1.00	1.19	1.64	0.84	–	1.25	0.78	0.88	0.94	0.94	1.12	0.91	1.32	1.04	0.84	1.12	0.86	1.12	0.88
	Met	0.97	1.11	1.17	0.82	1.38	0.80	–	1.09	0.96	0.86	0.77	0.86	1.11	0.75	0.98	1.11	0.91	0.65	0.93	1.46
	Ile	1.32	1.18	1.32	1.21	1.05	1.29	0.92	–	1.13	1.02	1.03	0.95	1.28	0.25	0.85	0.92	1.33	0.88	0.83	1.29
	Asn	1.19	1.04	0.96	1.27	0.71	1.13	1.04	0.88	–	1.00	0.89	0.88	0.76	1.33	0.89	0.92	1.07	1.01	0.98	0.72
	Lys	1.20	0.95	0.92	0.97	0.60	1.06	1.16	0.98	1.00	–	0.89	0.84	0.73	1.94	1.05	1.35	1.13	0.87	0.81	1.04
	Ser	1.09	1.18	0.91	1.22	1.02	1.06	1.30	0.97	1.13	1.13	–	1.17	0.91	1.10	1.08	1.05	1.14	1.04	1.05	1.22
	Thr	1.38	1.17	0.93	1.03	1.08	0.89	1.16	1.06	1.14	1.20	0.85	–	0.86	0.57	1.06	0.80	1.05	0.93	0.80	1.15
	Cys	1.12	1.24	0.93	1.62	1.03	1.10	0.90	0.78	1.31	1.36	1.09	1.16	–	0.93	0.84	1.18	1.00	0.76	0.93	1.00
	Trp	1.11	1.70	0.81	0.57	0.72	0.76	1.33	4.00	0.75	0.52	0.91	1.75	1.08	–	1.30	0.89	0.76	0.75	0.55	0.83
	Val	0.91	1.06	1.10	1.30	1.21	0.96	1.02	1.17	1.12	0.96	0.92	0.94	1.19	0.77	–	1.05	0.91	0.72	0.90	1.02
	Leu	0.83	1.04	1.08	1.18	0.99	1.19	0.90	1.09	1.09	0.74	0.95	1.25	0.84	1.12	0.95	–	0.98	1.39	1.10	0.94
	Glu	1.03	1.11	0.89	0.95	0.56	0.89	1.10	0.75	0.93	0.88	0.87	0.95	1.00	1.32	1.10	1.02	–	0.86	0.69	0.91
	Asp	1.14	1.35	1.03	1.29	1.07	1.17	1.55	1.14	0.99	1.15	0.96	1.08	1.32	1.33	1.38	0.72	1.16	–	0.81	0.83
	His	1.45	0.95	1.06	0.90	0.62	0.90	1.08	1.20	1.02	1.23	0.95	1.25	1.07	1.83	1.11	0.91	1.46	1.23	–	1.06
	Gln	1.25	0.83	1.13	0.87	0.87	1.13	0.69	0.78	1.38	0.96	0.82	0.87	1.00	1.21	0.98	1.06	1.09	1.21	0.94	–

Each element R_ij in the AARM represents the ratio of number of replacements of the residue i by the residue j in the forward direction (mouse → human) to that in the reverse direction. This means that if R_ij >1, the number of (i)_Mouse → (j)_Human replacements is higher than the number of (j)_Mouse → (i)_Human replacements and if R_ij <1, the reverse is true. Bold and Bold-italics ratios signifies the directional bias at p < 0.0001 and 0.001 respectively.