Table 4.
Top 15 amino acid pairs of three orthologous groups according to differences in number of forward (mouse to human) and reverse (human to mouse) replacements in AARM
| Pair | Forward no. | Reverse no. | Difference | Ratio | Codon changes | Trends |
|---|---|---|---|---|---|---|
| High-GC group | ||||||
| Thr → Ala* | 6873 | 4020 | 2853 | 1.71 | ACN → GCN | ± |
| Ser → Ala* | 4160 | 2447 | 1713 | 1.70 | UCN/AGY → GCN | ± |
| Ser → Pro* | 3603 | 2097 | 1506 | 1.72 | UCN/AGY → CCN | ± |
| Val → Ala* | 4484 | 3144 | 1340 | 1.43 | GUN → GCN | ± |
| Ser → Gly* | 3749 | 2516 | 1233 | 1.49 | UCN/AGY → GGN | + |
| Gln → Arg* | 3389 | 2448 | 941 | 1.38 | CAR → CGN/AGR | |
| Lys → Arg* | 3412 | 2518 | 894 | 1.36 | AAR → CGN/AGR | ± |
| Ile → Val* | 4572 | 3756 | 816 | 1.22 | AUH → GUN | ± |
| Asn → Ser* | 2878 | 2145 | 733 | 1.34 | AAY → UCN/AGY | ± |
| Asp → Glu* | 3799 | 3237 | 562 | 1.17 | GAY → GAR | • |
| His → Arg* | 1899 | 1343 | 556 | 1.41 | CAY → CGN/AGR | + |
| Met → Leu* | 1753 | 1211 | 542 | 1.45 | AUG → UUR/CUN | + |
| Ile → Leu* | 1500 | 1012 | 488 | 1.48 | AUN → UUR/CUN | ± |
| Lys → Glu* | 1257 | 785 | 472 | 1.60 | AAR → GAR | |
| Leu → Pro* | 2060 | 1634 | 426 | 1.26 | YYR/CUN → CCN | + |
| Medium-GC group | ||||||
| Val → Ile* | 5839 | 4982 | 857 | 1.17 | GUN → AUH | □ |
| Thr → Ala* | 5566 | 4753 | 813 | 1.17 | ACN → GCN | ○ |
| Ser → Pro* | 3804 | 3113 | 691 | 1.22 | UCN/AGY → CCN | ○ |
| Asp → Glu* | 4443 | 3833 | 610 | 1.16 | GAY → GAR | • |
| Ser → Thr* | 3652 | 3113 | 539 | 1.17 | UCN/AGY → ACN | □ |
| Ser → Ala* | 3507 | 2972 | 535 | 1.18 | UCN/AGY → GCN | ○ |
| Ser → Asn* | 3626 | 3213 | 413 | 1.13 | UCN/AGY → AAY | □ |
| Arg → Lys** | 4017 | 3701 | 316 | 1.08 | CGN/AGR → AAR | □ |
| Leu → Pro* | 1999 | 1691 | 308 | 1.18 | UUR/CUN → CCN | ○ |
| Gln → Arg* | 2676 | 2372 | 304 | 1.13 | CAR → CGN/AGR | ○ |
| Ser → Gly | 2782 | 2560 | 222 | 1.09 | UCN/AGY → GGN | ○ |
| Val → Ala | 3759 | 3561 | 198 | 1.06 | GUN → GCN | ○ |
| Lys → Glu** | 1514 | 1336 | 178 | 1.13 | AAR → GAR | ○ |
| Asp → Ala* | 661 | 488 | 173 | 1.35 | GSN → GCN | |
| Met → Leu | 1648 | 1483 | 165 | 1.11 | AUG → UUR/CUN | ○ |
| Low GC group | ||||||
| Val → Ile* | 9402 | 6335 | 3067 | 1.48 | GUN → AUH | ± |
| Ser → Asn* | 6508 | 3733 | 2775 | 1.74 | UCN/AGY → AAY | ± |
| Arg → Lys* | 6603 | 4059 | 2544 | 1.63 | CGN/AGR → AAR | ± |
| Ala → Thr* | 6393 | 5117 | 1276 | 1.25 | GCN → ACN | ± |
| Leu → Ile* | 3003 | 2035 | 968 | 1.48 | UUR/CUN → AUH | ± |
| Asp → Glu* | 5952 | 5014 | 938 | 1.19 | GAY → GAR | • |
| Ser → Thr* | 4498 | 3563 | 935 | 1.26 | UCN/AGY → ACN | |
| Ala → Val* | 4193 | 3264 | 929 | 1.28 | GCN → GUN | ± |
| Thr → Ile* | 2612 | 1733 | 879 | 1.51 | CAN → AUH | – |
| Pro → Ser* | 4178 | 3304 | 874 | 1.26 | CCN → UCN/AGY | ± |
| Ala → Ser* | 3975 | 3195 | 780 | 1.24 | GCN → UCN/AGY | ± |
| Leu → Phe* | 2990 | 2330 | 660 | 1.28 | UUR/CUN → UUY | – |
| Thr → Asn* | 1630 | 975 | 655 | 1.67 | CAN → AAY | |
| Met → Ile* | 1900 | 1288 | 612 | 1.48 | AUG → AUH | – |
| His → Asn* | 1258 | 715 | 543 | 1.76 | CAY → AAY | – |
*,**Replacements of pairs are significant at p < 0.0001 and 0.001, respectively.
Symbols used in codons: R, A/G; Y, C/T; S, G/C; W, A/T; H, A/C/T; N, any nucleotide. Symbols used in trends: opposite trends among top 15 amino acid pairs of both high- and low-GC groups (±); opposite trends in high- and low-GC group, but not present in top 15 of low-GC group (+); opposite trends in high- and low-GC group, but not present in top 15 of high-GC group (–); same trend in high-, medium-, and low-GC groups (•); same trend in high- and medium-GC groups (○); same trend in low- and medium-GC groups (□).