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. 2007 Oct 6;14(4):183–199. doi: 10.1093/dnares/dsm016

Table 4.

Influence of X-ray irradiation on the growth of F1 progeny from RNAi-treated animals

Body length (mm)
Control D2005.5 (RNAi) gei-17 (RNAi)
Mock irradiated 0.932 ± 0.062 (n = 40) 0.923 ± 0.076 (n = 32) 0.919 ± 0.116 (n = 43)
Significance relative to control (P-value) 0.521 0.560
X-ray irradiated (40 Gy) 0.992 ± 0.076 (n = 56) 0.726 ± 0.208 (n = 18) 0.826 ± 0.211 (n = 31)
Significance relative to control (P-value) <0.0001 <0.0001

The genomic DNA fragment corresponding to gei-17(W10D5.3) was amplified using the primer set W10D5.3-F (5′-CGCTTCCACTTCCATTCTACGATG-3′) and W10D5.3-R (5′-GGCCATTCCAGATGGAGATGAGCC-3′). The D2005.5 cDNA fragment (∼1.5 kb) was amplified from a C. elegans embryo cDNA library using the primers D1-BF (5′-CCGGGATCCATCGTTGATCTGATGCCTGCGATGG-3′) and ZAP-R (5′-GAATTGTAATACGACTCACTATAGGGC-3′). The D2005.5 cDNA and gei-17 genomic DNA fragment were used for an X-ray-induced growth retardation assay. The growth of larvae from RNAi-treated animals was monitored by determining the mean body length of the animals. The mean ± standard deviation values of body length of animals at 3 days after X-ray or mock irradiation were determined and are indicated . Numbers of animals measured are in parentheses. Statistical significance of the differences in mean body length between control and RNAi-treated animals in each group was analysed by Student's t-test (significance at P < 0.05) using the software package JMP IN5.1.2J (SAS Institute, Cary, USA).