Figure 7.

Ammonium chloride treatment. Cell lines stably expressing proCL, N2N3, or N2Q3 recombinants. Cells were radiolabeled for 5 h with [³⁵S]methionine in the absence (−) or presence (+) of NH₄Cl (10 mM). Cell lysates (C) and culture media (M) were collected and subjected to immunoprecipitation with anti-human proCL antiserum (last three panels). In the left panel (Mouse), anti-mouse proCL antiserum was used to immunoprecipitate endogenous protein from lysates of cells expressing the N2N3 recombinant, using 10-fold less sample than in the N2N3 panel. Positions of proenzymes and processed proteins (along with the number of carbohydrates on each protein) are indicated, as previously. The 29-kDa size standard is shown on the left and lane numbers are provided below each panel.