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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: FEBS J. 2007 Nov 1;274(23):6152–6166. doi: 10.1111/j.1742-4658.2007.06136.x

Figure 1.

Figure 1

Negative-ion ESI mass spectra of (A) fish pepsinogen A1 and (B) activated fish pepsin A1. The measured molecular weights were 38,845 Da for the pepsinogen form (theoretical mass: 38,850.6 Da) and 34,250 Da for the activated form (theoretical mass: 34,241.2 Da). The conversion of pepsinogen to pepsin leads to a mass shift of ~ 4600 Da due to the removal of the N-terminal residues 1–38. (C) Negative-ion ESI mass spectra of pig pepsin A from Sigma, as a control (theoretical mass: 34,583.8 Da; measured mass: 34,584 Da). (D). Commassie-stained SDS-PAGE gel of fish pepsin A1 before (lane 1) and after (lane 2) activation.