Table 1.
SNP | Primer Name | Primer Sequence (5' – 3') |
glnA36 | glnA36-C | ATATCCTGATTTAGATACTTGGATTCTC |
glnA36-T | ATATCCTGATTTAGATACTTGGATTCTT | |
glnA36-Rev | TCTCCTTCTGCTGTATAGATATCACA | |
glnA4291 | glnA429-G1 | TTGTTTTAACAACCAATTTAAATAATTGAATC |
glnA429-A | TTGTTTTAACAACCAATTTAAATAATTGAATT | |
glnA429-For | AAGTAGCAGTTGGACAGGATGAAA | |
glcK180 | glcK180-A | GGCTGATACTCAAGAAGTAGGTTAA |
glcK180-G | GGCTGATACTCAAGAAGTAGGTTAG | |
glcK180-Rev | ACCAAGTGCTGCAACATTAGC | |
adhP111 | adhP111-G | TTGCATGGTTCTTTGAATGG |
adhP111-A | TTGCATGGTTCTTTGAATGA | |
adhP111-T | TTGCATGGTTCTTTGAATGT | |
adhP111-Rev | CAAAGCGTCTCACGTCCTGT | |
atr351 | atr351-A | GTTGTTGTTGTCCTCCAGATAAGCTA |
atr351-T | GTTGTTGTTGTCCTCCAGATAAGCTT | |
atr351-Rev | GGACTCAAAGAGAAGGCTAATGCT |
The allele specific primers are named in accordance with their specificities, while the common primers are names in accordance with their orientation with respect to the coding DNA sequences.
1The glnA429 allele specific primers are in the reverse direction, hence the inconsistency between the alleles and the 3' terminal nucleotides.