Table 2.
Thermodynamic Parameters for Duplex Formation
| Duplex | Tm[a] (°C) | ΔH0 (kcal/mol) | ΔS0 (cal mol-1 K-1) | ΔG25°C (kcal/mol) | ΔΔG25°C (kcal/mol) |
|---|---|---|---|---|---|
| 5′-ATGGCXYGCTAT-3′ 3′-TACCGNMCGATA-5′ |
|||||
| 1 (XY/MN: GT/AC) | 60.1 | -83±4.8 | -220±13 | -16.2±0.48 | |
| 2 (XY/MN: GT/AA) | 43.1 | -72±4.5 | -200±13 | -11.6±0.21 | 4.6 |
| 3 (XY/MN: TG/CA) | 60.1 | -77±1.3 | -207±4 | -15.6±0.13 | 0.6 |
| 4 (XY/MN: G*T/AC) | 56.4 | -83±2.4 | -230±7 | -15.3±0.21 | 0.9[b] |
| 5 (XY/MN: G*T/AA) | 52.4 | -90±7.2 | -250±20 | -15.1±0.58 | -3.5[b] |
| 6 (XY/MN: GTg/AC) | 44.7 | -78±1.5 | -222±4 | -12.2±0.08 | 4.0[b] |
| 7 (XY/MN: G*Tg/AC) | 40.9 | -69±2.0 | -195±6 | -11.1±0.08 | 5.1[b] |
| 8 (XY/MN: G*Tg/AA) | 37.5 | -81±5.7 | -240±17 | -10.7±0.21 | 0.9[b] |
| 9 (XY/MN: TgG*/CA) | 37.4 | -76±5.0 | -220±15 | -10.5±0.15 | 5.1[b] |
[a]
Ct = 16 μM
[b]
ΔG25°C (modified ODN) - ΔG25°C (corresponding unmodified ODN).