Figure 3.
Expression of Ca2+ pumps restores growth of a pmr1 mutant on EGTA-containing media. (A) Expression of the vacuolar ion pump Pmc1 in pmr1 cells. Serial fivefold dilutions of saturated cultures were spotted onto complete medium lacking uracil to maintain selection for the plasmids. Addition (+) or omission (−) of 6.5 mM EGTA is indicated. Plates were photographed after 3 d incubation at 30°C. Strains (from left to right): YR439 (pmr1; 2μ-vector); YR657 (pmr1, 2μ-PMR1) and YGD57 (pmr1, 2μ-PMC1). (B) Expression of a sarcoplasmic Ca2+ pump, SERCA1a (rabbit). Conditions as in panel A; strains are (from left to right): YR441 (pmr1, 2μ-PMR1), YR439 (pmr1, 2μ-vector), YR663 (pmr1, CEN-SERCA1a), and YR664 (pmr1, 2μ-SERCA1a).