Table 1. Genetic map distances (cM) and the distribution of parental and recombinant progeny for the EAY1108/EAY1112 strain background in WT, csm4Δ, ndj1Δ, msh5Δ, and mlh1Δ strains on chromosome XV.
| Relevant genotype | Tetradsa | Single sporesb | |||||||
| Number analyzed | cM | PD | TT | NPD | Number analyzed | cM | Parental | Recombinant | |
| URA3-LEU2: | |||||||||
| wild-type | 1068 | 21.8–23.8 | 607 | 456 | 5 | 4644 | 20.6–23.0 | 3635 | 1009 |
| csm4Δ | 531 | 33.3–36.9 | 203 | 319 | 9 | 2999 | 29.3–32.6 | 2072 | 927 |
| ndj1Δ | 472 | 34.9–39.1 | 173 | 289 | 10 | 2548 | 31.0–34.7 | 1712 | 836 |
| mlh1Δ | 616 | 10.3–12.5 | 486 | 128 | 2 | 3792 | 9.6–11.6 | 3393 | 399 |
| msh5Δ | 720 | 5.0–6.4 | 643 | 76 | 1 | 5674 | 5.1–6.3 | 5352 | 322 |
| csm4Δ ndj1Δ | 789 | 31.8–35.0 | 337 | 437 | 15 | 3836 | 27.4–30.3 | 2732 | 1104 |
| csm4Δ mlh1Δ | 418 | 15.4–19.2 | 298 | 115 | 5 | 4036 | 13.6–15.8 | 3446 | 590 |
| csm4Δ msh5Δ | 155 | 7.5–10.5 | 127 | 28 | 0 | 1624 | 8.2–11.1 | 1469 | 155 |
| LEU2-LYS2: | |||||||||
| wild-type | 1068 | 26.6–28.4 | 496 | 569 | 3 | 4644 | 25.8–28.4 | 3388 | 1256 |
| csm4Δ | 531 | 29.7–32.5 | 216 | 312 | 3 | 2999 | 28.0–31.3 | 2110 | 889 |
| ndj1Δ | 472 | 31.3–34.3 | 192 | 274 | 6 | 2548 | 28.3–31.9 | 1782 | 766 |
| mlh1Δ | 616 | 11.8–13.6 | 459 | 157 | 0 | 3792 | 11.7–13.8 | 3309 | 483 |
| msh5Δ | 720 | 11.0–13.0 | 562 | 155 | 3 | 5674 | 10.3–11.9 | 5047 | 627 |
| csm4Δ ndj1Δ | 789 | 31.9–34.9 | 322 | 455 | 12 | 3836 | 29.1–32.0 | 2664 | 1172 |
| csm4Δ mlh1Δ | 418 | 14.4–17.4 | 295 | 121 | 2 | 4036 | 14.5–16.7 | 3407 | 629 |
| csm4Δ msh5Δ | 155 | 10.4–15.4 | 120 | 34 | 1 | 1624 | 10.0–13.2 | 1437 | 187 |
| LYS2-ADE2: | |||||||||
| wild-type | 1068 | 12.1–13.7 | 803 | 263 | 2 | 4644 | 11.8–13.8 | 4052 | 592 |
| csm4Δ | 531 | 15.3–17.5 | 362 | 168 | 1 | 2999 | 16.0–18.7 | 2480 | 519 |
| ndj1Δ | 472 | 17.8–20.0 | 294 | 178 | 0 | 2548 | 16.6–19.7 | 2087 | 461 |
| mlh1Δ | 616 | 6.2–7.6 | 531 | 85 | 0 | 3792 | 6.5–8.1 | 3517 | 275 |
| msh5Δ | 720 | 3.7–4.7 | 659 | 61 | 0 | 5674 | 4.1–5.3 | 5409 | 265 |
| csm4Δ ndj1Δ | 789 | 18.7–21.3 | 514 | 267 | 8 | 3836 | 16.8–19.3 | 3145 | 691 |
| csm4Δ mlh1Δ | 418 | 6.1–7.7 | 360 | 58 | 0 | 4036 | 6.9–8.6 | 3726 | 310 |
| csm4Δ msh5Δ | 155 | 3.1–5.3 | 142 | 13 | 0 | 1624 | 3.1–5.1 | 1559 | 65 |
All mutants are isogenic derivatives of EAY1108/EAY1112. aIntervals correspond to the genetic distance calculated from tetrads +/- one standard error. Standard error was calculated using the Stahl Laboratory Online Tools website (http://www.molbio.uoregon.edu/fstahl/). bData shown as 95% confidence intervals around the recombination frequency determined from single spores. To facilitate comparisons to the tetrad data, recombination frequencies obtained from single spore data were multiplied by 100 to yield genetic map distances (cM). The recombination frequency in single spores determined by: Parental/(Parental+Recombinant) and cM indicates the genetic distance in tetrads calculated using the formula of Perkins [38]: 50×{TT+(6×NPD)}/(PD+TT+NPD).