Fig. 5.

Effects of buffer pH on competitive binding of CoQ10, α-Toc, and γ-Toc to saposin B. Each lipid (10 mM) in hexane solution was incubated with aqueous 0.5 µM saposin B for 5 min. All experiments were repeated 3 times and means ± SD are shown. *, **, and *** indicate significant differences (p<0.05, 0.01 and 0.001, respectively) compared to values for γ-Toc.